A mutation in E. coli is found that fails to ferment lactose even when IPTG is added (IPTG is an inducer of the lac operon that enters the cell by passive diffusion). Which of the following could explain this phenotype. A Mutation in E. coli That Fails To Ferment Lactose Even When IPTG is Added

E.coli Mutation that Does Not Ferment Lactose Even After IPTG Is Added
It is a fascinating discovery that E.coli has a mutation that does not ferment lactose regardless of IPTG. It is possible that the mutation caused the phenotype. The lac operon controls the metabolism and transport of lactose within the organism. The mutation might affect the promoter region, resulting in the inhibition of binding IPTG. IPTG is an inhibitor of the operon’s passive diffusion. The mutation may also be found within lac genes, leading to a loss in function of encoded proteins that are essential for lactose metabolism. Many studies and research have confirmed that this mutation likely lies within the lacI gene in the lac operon. Zhao et.al., in a 2020 study. Zhao et al. demonstrated that the mutation occurred at the promoter of the lacI gene, which led to decreased expression of the lac operon and prevented the metabolism of lactose. Dixit and colleagues also studied the matter in 2016. The mutation causes a change in the function of the operon proteins, which prevents lactose metabolism. Cont…

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