A student obtained Arabidopsis mutant jar-1 and found that the mutant was insensitive to JA. The addition of JA could not slow down the root growth of jar 1, and he also found that the content of JA-Ile was low in the mutant plants. JA-Ile can effectively inhibit the growth of roots, so he speculated that it might lose the conjugate enzyme activity resulting in a low content of JA-Ile. What experiments can he do to confirm his presumption? The Role of JAR1 in JA-Ile Biosynthesis and Its Effect on Arabidopsis Root Growth

The Position of JAR1 in JA-Ile Biosynthesis and Its Impact on Arabidopsis Root Progress

The coed’s speculation that JAR1 mutant crops could have decreased JA-Ile ranges on account of a scarcity of conjugate enzyme exercise might be examined by just a few experiments. First, the coed can measure the JA-Ile ranges within the JAR1 mutant and examine it to the wild-type management. If there’s a vital distinction between the 2, it will counsel a distinction in JA-Ile biosynthesis (Maruyama et al., 2016). Second, the coed can measure the degrees of JA within the JAR1 mutant and wild-type management. If the degrees of JA are considerably completely different, it will counsel a distinction in JA-Ile biosynthesis. Third, the coed can measure the expression ranges of the JAR1 gene within the JAR1 mutant and wild-type management. If the degrees of expression are considerably completely different, it will counsel a distinction in JA-Ile biosynthesis (Niu et al., 2017). Cont…