Measuring Absorbance of an Enzyme Using Spectrophotometry with Butanol
When measuring the absorbance of an enzyme using spectrophotometry, it is important to use an appropriate blank sample. A blank sample is a solution of the same solvent used in the experiment, which contains no analyte. In this case, the solvent used for the enzyme is butanol. To use this as the blank sample, the technician should prepare a solution of butanol and place it in the spectrophotometer. This blank sample will then be used to measure the absorbance of the enzyme solution (Rutledge, 2019). Since the absorbance is affected by the concentration of the enzyme, the technician should ensure that the blank sample has the same concentration as the enzyme solution. This can be accomplished by diluting the enzyme solution with butanol until the desired concentration is reached. The technician should then compare the absorbance of the blank sample with that of the enzyme solution in order to calculate the absorbance (Kisiel et al., 2020). Cont…
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