A technician wants to measure the absorbance of an enzyme using spectrophotometry. The enzyme is dissolved in butanol. What should the technician use as a blank. Measuring Absorbance of an Enzyme Using Spectrophotometry with Butanol

Measuring Absorbance of an Enzyme Using Spectrophotometry with Butanol

When measuring the absorbance of an enzyme using spectrophotometry, it is important to use an appropriate blank sample. A blank sample is a solution of the same solvent used in the experiment, which contains no analyte. In this case, the solvent used for the enzyme is butanol. To use this as the blank sample, the technician should prepare a solution of butanol and place it in the spectrophotometer. This blank sample will then be used to measure the absorbance of the enzyme solution (Rutledge, 2019). Since the absorbance is affected by the concentration of the enzyme, the technician should ensure that the blank sample has the same concentration as the enzyme solution. This can be accomplished by diluting the enzyme solution with butanol until the desired concentration is reached. The technician should then compare the absorbance of the blank sample with that of the enzyme solution in order to calculate the absorbance (Kisiel et al., 2020). Cont…

Calculate the price of your order

550 words
We'll send you the first draft for approval by September 11, 2018 at 10:52 AM
Total price:
The price is based on these factors:
Academic level
Number of pages
Basic features
  • Free title page and bibliography
  • Unlimited revisions
  • Plagiarism-free guarantee
  • Money-back guarantee
  • 24/7 support
On-demand options
  • Writer’s samples
  • Part-by-part delivery
  • Overnight delivery
  • Copies of used sources
  • Expert Proofreading
Paper format
  • 275 words per page
  • 12 pt Arial/Times New Roman
  • Double line spacing
  • Any citation style (APA, MLA, Chicago/Turabian, Harvard)

Our guarantees

Delivering a high-quality product at a reasonable price is not enough anymore.
That’s why we have developed 5 beneficial guarantees that will make your experience with our service enjoyable, easy, and safe.

Money-back guarantee

You have to be 100% sure of the quality of your product to give a money-back guarantee. This describes us perfectly. Make sure that this guarantee is totally transparent.

Read more

Zero-plagiarism guarantee

Each paper is composed from scratch, according to your instructions. It is then checked by our plagiarism-detection software. There is no gap where plagiarism could squeeze in.

Read more

Free-revision policy

Thanks to our free revisions, there is no way for you to be unsatisfied. We will work on your paper until you are completely happy with the result.

Read more

Privacy policy

Your email is safe, as we store it according to international data protection rules. Your bank details are secure, as we use only reliable payment systems.

Read more

Fair-cooperation guarantee

By sending us your money, you buy the service we provide. Check out our terms and conditions if you prefer business talks to be laid out in official language.

Read more