An important question about capped eukaryotic messenger RNA is whether the cap location is a processing site produced by cleavage from a longer RNA or whether the cap site is the actual start site of transcription. How could _-labeled ATP be used in vitro to answer this question for an RNA that begins GpppApXp? How could you determine the average physical half-life of mRNA in cells from data obtained by simultaneously adding radioactive uridine and an inhibitor of the initiation of RNA synthesis?
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