The Impact of Centrifugation on Cell Sample Composition: How Increasing Centrifugation Affects Charge of Components in the Pellet
Centrifugation is a technique used to separate different components of a homogenised cell sample. By increasing the rate of centrifugation, the relative proportions of the components in the pellet become more concentrated. The resulting change in composition leads to an accompanying change in the charge of the components in the pellet. This has been demonstrated through a variety of biochemical studies. One such study, conducted by G.J. Niedermeier et al. (2017), showed that increasing centrifugation at a constant temperature led to a greater concentration of negatively charged components in the pellet. This was attributed to the larger particles becoming more tightly packed, leading to a greater concentration of negatively charged components. Similarly, a study by S.G. Fridman (2020) found that increased centrifugation of a cell sample led to a decrease in the magnitude of the overall charge of the components in the pellet. This was attributed to the increased concentration of the larger particles leading to a greater overall negative charge. Cont…
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