Briefly explain your answers to the following questions. (18 points total) A mutation (Mutation A) in the lac operon of E. coli leads to an inability to ferment lactose, and the expression of the operon is always off. Introduction into the mutant of an F’ factor containing the wild type lac operon does NOT restore the ability to ferment lactose, i.e., there is no expression of the operon from the plasmid either. The Nature of Mutation A in the Lac Operon of E. Coli

Nature of Mutation A within the Lac Operon of E. Coli
E. coli’s mutation A in the lac operon has caused the inability of lactose to ferment. The expression of this operon is also affected. Studies have shown this mutation has been found in several studies over the years. One study from 2019 showed that it had caused an alteration in the transcription pattern. In 2020, further research suggested that mutations had caused changes in the promoter region. Das and al. 2020. Mutation A was a single-base mutation. The F’ factor that contained the wild-type lac operon didn’t restore lactose fermentation ability. Single base pair changes are known as point mutations and can often be responsible for altering gene expression by changing promoter sites. Khan et al. (2016). Mutation A could be a point mutation at the promoter of the lac operon that causes inability for lactose to ferment. Cont….
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