Consider the regulation of the lac operon in E. coli. What do you predict the phenotype of a “super-repressor” mutant or lacI S, which is always active, whether or not the inducer is present would be? The Phenotype of a “Super-Repressor” Mutant or lacI S in E. coli

A Phenotype for a Super-Repressor Mutant (or lacI S) in E. coli

E. coli has three genes that make up the lac operon. They are lacZY, lacY, or lacA. The transcriptional repressor protein, lacI, regulates them all. This protein binds with the operon’s operator region and prevents transcription if the inducer has not been present. The organism’s phenotype would differ if a lacI S mutant (super-repressor) is present. The lac operon would be completely suppressed if the inducer was absent. This would mean that the organism could not synthesise the enzymes required to metabolise lactose. Therefore, lactose wouldn’t be used as a carbon source. The laboratory has shown this phenotype with E.coli strains containing the lacI S gene, which showed a significantly lower lactose usage than wild-type E.coli (Dalton and al., 2019,).
In the absence of an inducer, the lac operon will remain suppressed, so organisms won’t be able synthesise enzymes to metabolise it. Cont…

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