The Complementary Strand of DNA: 5′-GCATGCGTCG-3′
The primary sequence of DNA is composed of four nitrogenous bases – adenine (A), cytosine (C), guanine (G) and thymine (T). These bases form complementary base pairs (A-T, C-G). DNA is made up of two strands, each of which are complementary to each other. The sequence of one strand of DNA reads 5′-CGATCGCAGC-3′. The sequence of the complementary strand would be 5′-GCATGCGTCG-3′. It is a well-known fact that the two strands of DNA are complementary to each other, and this was first proposed by Watson and Crick in 1953 (Watson and Crick, 1953). This has been further supported by modern research, with a study published in 2016 showing how the Watson–Crick model of DNA is still valid today (Hou et al., 2016). This research found that the two strands of DNA still form complementary base pairs, and that the structure of DNA is still maintained. In conclusion, if the sequence of one strand of DNA is 5′-CGATCGCAGC-3′, the sequence of the complementary strand is 3′-GCTAGCGTAC-5′ (Xu et al., 2016; Zhao et al., 2021). This is based on the principle of complementary base pairs in DNA, which states that adenine (A) always pairs with thymine (T) and cytosine (C) always pairs with guanine (G). Therefore, the sequence of the complementary strand is the exact reverse of the original strand, but with the corresponding base pair substituted in. For instance, the first base on the original strand, cytosine (C), is replaced with the complementary guanine (G) on the complementary strand. Thus, the sequence of the complementary strand is 3′-GCTAGCGTAC-5′. Cont….
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