The Significance of a P-Value of 103 in a BLAST Search of the Drosophila Genome
A BLAST (Basic Local Alignment Search Tool) search of the Drosophila genome using the human RB protein sequence as a query can yield a Subject sequence with a p-value of 103. A p-value is a measure of the probability that the similarity between the query and Subject sequence is due to chance. Therefore, a p-value of 103 indicates a low probability of similarity between the two sequences arising from chance. However, the exact interpretation of the p-value depends on the threshold set for the search (Jiang et al., 2016). In general, a p-value of 103 indicates that the query and Subject sequence are significantly similar and that the Drosophila genome contains a sequence that is homologous to the human RB protein sequence. This is further supported by the fact that the p-value is below the threshold set for the search. Additionally, the similarity between the two sequences suggests that they share a common ancestor and that the human RB protein sequence has evolved from a sequence present in the Drosophila genome (He et al., 2021). Cont…
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