Next generation sequencing (NGS) has significantly decreased the cost and increased the speed and efficiency with which we sequence DNA. a. Choose one NGS methodology: i. Describe the principle behind the NGS methodology. The principle behind the Next Generation Sequencing (NGS)

Next Generation Sequencing is the basic principle of Next Generation Sequencing.
Next Generation Sequencing (NGS), a method of Illumina Sequencing, uses the principle of bridge amplification to produce short DNA fragments (150 to 300 base pairs), which can then be sequenced (Mallick and al. 2017,). First, short fragments are ligated to adapters. They then get separated into separate clusters and put in a flow cell. The DNA fragments can then be amplified and made into millions of duplicates of the original. Next, the fragments of DNA are sequenced with fluorescently labeled nucleotides. These can be detected by Illumina’s sequencer (Singh and al., 2020). To create a sequence, the sequencing process is repeated millions upon millions of times. It produces a large amount of high-quality data that can be used to identify microbial species, as well as gene expression analysis and variant detection (DeJesus and al., 2019). Cont…..
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