Phosphorus’s Pathway from Radioactivity to Activating His Residue in Phosphoglycerate Mutase
Radioactive phosphorus can be used to label the atom of the phosphate group that activates His residue in phosphoglycerate mutase. This process begins with the introduction of the phosphorus atom into a molecule of phosphorus-32, which is a radioactive isotope of phosphorus (Chrétien et al., 2020). This isotope of phosphorus is then incorporated into molecules that act as a substrate for the enzyme, phosphoglycerate mutase (PGM). Next, the enzyme acts upon the substrate, cleaving the phosphate group and transferring the phosphorus atom to the His residue. This process activates the His residue and allows it to act as a substrate for other enzymes (Meyer et al., 2016). Finally, the activated His residue can then bind to the substrate molecules and act as a catalyst for the phosphorylation of molecules. This phosphorylation process is essential for the production of high energy molecules that can be used in a variety of metabolic processes (Khan et al., 2021). Thus, the pathway of phosphorus from radioactivity to activating His residue in phosphoglycerate mutase is outlined above. Cont….
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