The concentration of lactate is determined by measuring the amount of NADH produced. At what wavelength (in units of nanometres, nm) can we measure the absorbance of this chromogen? Measuring the Absorbance of NADH Using Wavelengths of Light

Measuring the Absorbance of NADH Using Wavelengths of Light

The concentration of lactate is determined by measuring the amount of NADH produced. NADH is a chromogen, meaning it absorbs certain wavelengths of light. By measuring the intensity of the light, one can determine the amount of NADH present. The absorbance of NADH is typically measured between 340 to 375 nanometres (nm) (O’Farrell et al., 2016). This range is also sometimes referred to as the “visible” range of light. The absorbance of NADH can vary depending on the type of solution it is in. For example, if NADH is in an acidic solution, the absorbance will be higher than if it is in a neutral solution (Li et al., 2021). In addition, the absorbance of NADH can also be affected by the concentration of other molecules in the solution, such as glucose or lactate (O’Farrell et al., 2016). In summary, the absorbance of NADH can be measured by using light of specific wavelengths between 340 to 375 nm. Depending on the solution it is in, the absorbance of NADH may vary. Therefore, it is important to be aware of the solution and its components when measuring the absorbance of NADH. Cont….