The Molecular Nature and Clinical Phenotypical Manifestations of Quantitative PCR
Quantitative polymerase chain reaction (qPCR) is a powerful technique that has become increasingly sophisticated over the years and is used for a variety of biomedical applications, including the diagnosis of infectious diseases, cancer, genetic mutations, and other genetic disorders (Rizvi et al., 2016). qPCR relies on the use of specific primers and fluorescent probes to amplify and detect the presence of a target DNA or RNA sequence. Since its inception, it has become an invaluable tool for the detection and quantification of nucleic acid sequences, allowing for the rapid and sensitive detection of a wide range of diseases and disorders (Vorontsova et al., 2020). In terms of the molecular nature of the disease, qPCR is used to detect and quantify the presence of a particular DNA or RNA sequence, which can be used to identify and quantify a wide range of mutations and genetic disorders (Do et al., 2021). For example, qPCR can be used to detect and quantify the presence of a particular gene mutation, which can be used to help diagnose and monitor certain diseases. Cont….
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