What is heterologous probing and what is it used for? 17 (5 points): Calculate the melting temperature (T m ? ) of the below primer sequence 5′ ACCGTTAAGCTGACGCC 3′ Heterologous Probing: Definition, Uses and Benefits

Heterologous Probing: Definition, Uses and Benefits

Heterologous probing is a method used in biotechnology to identify the sequence of a gene and its use in the study of gene regulation. It is used to identify the sequence of a gene by using the complementary strand of a related gene to identify the base pairs of the gene being studied. This technique has been used in a variety of applications, including identifying transcriptional regulatory elements, predicting gene structure, and determining gene function. Heterologous probing is used to identify the base pair sequence of a gene by using a related gene. It uses the complementary strand of the related gene to identify the sequence of the gene being studied (Liao et al., 2017). This process is known as hybridization, which involves the binding of the complementary strands of the two genes. This can be done through the use of specific probes that bind to the target gene, or through the use of modified nucleotides that are complementary to the target gene. The hybridization of the two strands then allows for the identification of the target gene’s sequence. Heterologous probing is also used in the study of gene regulation. This technique can be used to identify transcriptional elements such as promoters and enhancers, which control gene expression. Additionally, it can be used to predict gene structure and determine gene function (Marzec & Strza?ka, 2018). Cont…

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