Exon Amplification: A Primer on the Process and Its Benefits
Exon amplification is a process used to increase the expression of genes by amplifying the coding regions, known as exons, of the gene. It is a powerful tool for molecular biologists, as it can be used to study gene expression, identify mutations, and even produce recombinant proteins. In recent years, exon amplification has become increasingly popular due to its ability to be used in a variety of applications (Rutherford et al., 2020). Exon amplification is a process that utilizes the polymerase chain reaction (PCR) to copy and amplify specific regions of a gene. The process begins with the identification of the specific exon that needs to be amplified. A primer is then designed to bind to the DNA sequence at either end of the exon. The PCR then amplifies the sequence, creating multiple copies of the exon (Kumar et al., 2018).
Exon amplification has several benefits. It allows researchers to identify mutations that may be responsible for certain diseases or traits. It also allows for the expression and production of recombinant proteins. Finally, it can be used to study gene expression and even detect epigenetic changes (Goncalves et al., 2016). Cont…..
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